Peptides are widely used in many fields, such as medicine, materials, dyes, and so on. The solubility of peptides is also an important factor for the success of laboratory experiments.Generally, the solubility of the peptide is usually closely related to the amino acid sequence of the peptides chain. Alkaline solution usually can be added to the peptides with Significant acidity which is difficult to dissolve, meanwhile acid solution should be added to the peptides with significant alkalinity which is difficult to dissolve .A small amount of organic solvent ,such as acetonitrile, DMSO, DMF, propyl alcohol, isopropyl alcohol and so on,can be added to the peptides which contains a large number of hydrophobic amino acid or neutral amino acids in the sequences. These peptides are difficult to directly dissolve in water. When the peptide containing Met or Cys, we can not dissolve it by DMSO .If so, it is likely to lead to the oxidation of S. We tend to give customers some effective suggestions bellows.
According to customers’ requirements, we usually give the following recommendations to increase the solubility of the insoluble peptide:
3.Replacing hydrophobic amino acid residues of sequences: increasing solubility effect by substituting soluble amino acid for hydrophobic amino acid in sequence. We usually use Gly, ala to replace the hydrophobic amino acids, which usually gets good dissolving effect.
Sequence:Biotin-GGGGGGGG
HPLC Analyse:
1. C term and the N term modification: for acidic peptides, N terminal amidation modification, for Alkaline peptides, C terminal acetylation.
2.Revision sequence of the peptide chain.: some sequence has a large number of continuous hydrophobic amino acids, such as W, F, V, I, l, m, y, these hydrophobic amino acid sequence at large or continuous repetition and whole peptide usually is insoluble,.In order to enhance the polarity and hydrophilicity of peptide, we also will advise clients to extend sequence or reduce the number of hydrophobic amino acids to increase solubility on the condition of non affecting experimental results.
Professional technical team, excellent equipment, skillful synthesis and purification process, Bankpeptide can provide various insoluble peptide conditions and capabilities. Reducing the experimental period for customers, improving the efficiency and achieving better results for customers’ research are always our company's service pursuit and aims.
Successful case:
MS Analyse:
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